1、目录下多个xls文件转换

import win32com.client as win32
import os
# 另存为xlsx的文件路径
xlsx_file = r"C:\Users\HP\Desktop\新建文件夹\xlsx"
xls_file = r"C:\Users\HP\Desktop\新建文件夹"
for file in os.scandir(xls_file):

    suffix = file.name.split(".")[-1]
    if file.is_dir():
        pass
    else:
        if suffix == "xls":
            excel = win32.gencache.EnsureDispatch('Excel.Application')
            wb = excel.Workbooks.Open(file.path)
            # xlsx文件夹路径\\文件名x
            wb.SaveAs(xlsx_file +"\\"+ file.name + "x", FileFormat=51)
            wb.Close()
            excel.Application.Quit()

2、增加界面化组件，让用户自行选择文件夹

import os
import win32com.client as win32
import easygui as eg

def pick_package():
    # 打开windows窗口，选择一个文件夹,并返回文件夹路径
    return eg.diropenbox()

if __name__ == "__main__":
    package = pick_package()  # 返回文件夹路径
    xlsx_file = package + "\\xlsx"
    for file in os.scandir(package):
        # 获取后缀
        suffix = file.name.split(".")[-1]
        if file.is_dir():  # 忽略文件夹
            pass
        else:
            if suffix == "xls":
                excel = win32.gencache.EnsureDispatch('Excel.Application')
                wb = excel.Workbooks.Open(file.path)
                wb.SaveAs(xlsx_file + "\\" + file.name + "x", FileFormat=51)
                wb.Close()
                excel.Application.Quit()

 自动创建文件夹

import os
import win32com.client as win32
import easygui as eg

def pick_package():
    # 打开windows窗口，选择一个文件夹,并返回文件夹路径
    return eg.diropenbox()

if __name__ == "__main__":
    package = pick_package()  # 返回文件夹路径
    # 在当前文件夹下创建xlsx文件夹，用于存放转换的文件
    if not os.path.exists(package+"\\" + "xlsx"):
        os.mkdir(package+"\\" + "xlsx")
    xlsx_file = package+"\\" + "xlsx"
    print(xlsx_file)
    for file in os.scandir(package):
        # 获取后缀
        suffix = file.name.split(".")[-1]
        if file.is_dir():  # 忽略文件夹
            pass
        else:
            if suffix == "xls":
                excel = win32.gencache.EnsureDispatch('Excel.Application')
                wb = excel.Workbooks.Open(file.path)
                wb.SaveAs(xlsx_file + "\\" + file.name + "x", FileFormat=51)
                wb.Close()
                excel.Application.Quit()

xlsx转xls

import os
import win32com.client as win32
import easygui as eg

def pick_package():
    # 打开windows窗口，选择一个文件夹,并返回文件夹路径
    return eg.diropenbox()

if __name__ == "__main__":
    package = pick_package()  # 返回文件夹路径
    # 在当前文件夹下创建xls文件夹，用于存放转换的文件
    if not os.path.exists(package+"\\" + "xls"):
        os.mkdir(package+"\\" + "xls")
    xlsx_file = package+"\\" + "xls"
    for file in os.scandir(package):
        # 获取后缀
        suffix = file.name.split(".")[-1]
        # 如果后缀为xlsx，执行xlsx转xls
        if suffix == "xlsx":
            excel = win32.gencache.EnsureDispatch('Excel.Application')
            wb = excel.Workbooks.Open(file.path)
            name = file.name.split(".")[0]
            wb.SaveAs(xlsx_file + "\\" + name, FileFormat=56)
            wb.Close()
            excel.Application.Quit()

SaveAs另存为xlsx时需补后缀x，另存为xls时仅路径和文件名即可